Proteometer-CV: A Technical Q&A for the Charge Variant Analysis Kit
- rsimpson473
- 41 minutes ago
- 3 min read
Today, we’re sitting down with Jess Westland, Product Manager, to discuss the newly launched Proteometer-CV Kit. This technology is set to revolutionize the way scientists conduct charge variant (CV) analysis in monoclonal and bispecific antibody development. Let’s take a deep dive into this exciting new product!
Q: Jess, thank you for joining us today. To begin, can you explain what the “CV” in Proteometer-CV stands for?
A: Absolutely. CV stands for Charge Variant. Analyzing charge variants in the drug development process is common practice, as they can drastically impact the final therapeutic product's efficacy, safety, and stability.
We are not introducing a new concept; we are simply introducing a new way to monitor these charge variants that is superior to current methods. The Proteometer-CV is the only solution that performs analysis straight from clarified fermentation broth, saving time and reducing variability due to sample handling.
Q: Who is the Proteometer-CV Kit designed for?
A: The Proteometer-CV Kit was developed specifically for scientists who develop monoclonal antibodies, bispecific antibodies, or fusion proteins with a human Fc region.
The assay has a wide range of applications throughout various phases of drug development, from discovery to preclinical testing and beyond. Whether you're focused on early-stage research or in the later phases of development, this kit can be a significant asset to your lab.
Q: From a scientific perspective, what exactly is the Proteometer-CV?
A: Scientifically speaking, the Proteometer-CV is a new and highly innovative analytical technology sold as a kit that plugs into any dual pump HPLC equipped with a fluorescence detector.
The capability of the kit to perform this CV analysis directly from clarified fermentation broth without extensive purification steps sets our product apart from others in the market. This attribute is critical because it preserves the sample's integrity, delivering accurate and reliable results faster than traditional methods.
Q: What problem does the Proteometer-CV solve, and how is it unique compared to other CV methods?
A: The primary problem the Proteometer-CV solves is the inefficiency and complexity associated with traditional charge variant analysis methods, such as capillary electrophoresis. These methods typically require purification steps such as Protein A affinity purification, which are time-consuming and resource intensive. The Proteometer-CV is unique because it eliminates these preparatory steps, allowing for rapid analysis directly from clarified fermentation broth. With a total cycle time of just 25 minutes, it significantly reduces both time and cost, making it a unique and highly efficient solution on the market.
Q: Could you walk us through how the Proteometer-CV Kit works?
A: Of course! The Proteometer-CV Kit includes everything you need for seamless charge variant analysis: a specialized reactor, a derivatizer module (MD), buffer packets, fluorescent reagent, tubing unions, and detailed instructions. The process is simple—you set up the assay on your HPLC system with the reagents and components provided, inject your samples onto the system, and within 25 minutes, you’ll have your results.
Q: In what phases of drug development can the Proteometer-CV Kit be utilized?
A: The kit is incredibly versatile and can be used throughout nearly all phases of drug development through process R&D. Its adaptability across different stages of development makes it an indispensable tool for scientists working with biologics.
Q: How has the technology behind the Proteometer-CV Kit been tested?
A: The technology has undergone extensive internal qualification. We’ve seen consistent results that match the performance of traditional methods. Furthermore, early adopters have reported significant improvements in their workflows, highlighting the speed, accuracy, and ease of use of the Proteometer-CV Kit. This robust qualification ensures that our technology delivers on its promises.
Q: Lastly, do you offer either virtual or on-site demos for potential users?
A: Of course! We understand that adopting new technology can be a significant decision, so we offer virtual and on-site demos to help potential users see the Proteometer-CV Kit in action. These demos allow us to walk through the setup, operation, and data analysis, providing a hands-on experience that showcases the kit's full capabilities.
We thank Jess for her time and ask those interested in scheduling a demonstration or asking additional questions to reach out to her directly at jwestland@novilytic.com.